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Identification and characterization of two novel human papillomaviruses (HPVs) by overlapping PCR: HPV102 and HPV106

¹ Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY, USA
² Division of Cancer Epidemiology and Genetics, National Cancer Institute, National Institutes of Health, US Department of Health and Human Services, Bethesda, MD, USA
³ Proyecto Epidemiológico Guanacaste, Costa Rican Foundation for Health Sciences, San José, Costa Rica
⁴ Department of Pediatrics, Department of Epidemiology and Population Health, Department of Obstetrics, Gynecology and Woman's Health and Albert Einstein Cancer Center, Albert Einstein College of Medicine, Bronx, NY, USA

Correspondence
Robert D. Burk
burk{at}aecom.yu.edu

Complete genomes of HPV102 (8078 bp) and HPV106 (8035 bp) were PCR amplified and cloned from cervicovaginal cells of a 49-year-old Hispanic female with reactive changes on her Pap test and a 42-year-old Hispanic female with a Pap test diagnosis of atypical squamous cells of unknown significance (ASCUS), respectively. The nucleotide sequence similarity of the complete L1 open reading frame (ORF) determined that HPV102 and HPV106 are most closely related to HPV83 (84.1 % identity) and HPV90 (83.5 % identity), respectively, placing them in the genital HPV groups, papillomaviruses species 3 and 15. HPV102 and HPV106 contain five early genes (E6, E7, E1, E2, and E4) and two late genes (L2 and L1), and both lack an E5 ORF. On the basis of phylogenetic analyses and available clinical information, these two novel HPV types expand the heterogeneity of HPVs detected in the lower genital tract.

Location of the predicted ORFs of HPV102 and HPV106 and sequence of the primers used are available as supplementary material with the online version of this paper.