HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Supplementary table and figure Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Shimada-Beltrán, H. Articles by Rivera-Bustamante, R. F. Search for Related Content PubMed PubMed Citation Articles by Shimada-Beltrán, H. Articles by Rivera-Bustamante, R. F. Agricola Articles by Shimada-Beltrán, H. Articles by Rivera-Bustamante, R. F.

Early and late gene expression in pepper huasteco yellow vein virus

Departamento de Ingeniería Genética, Cinvestav-Unidad Irapuato, km 9.6 Libramiento Norte, Apartado Postal 629, 36500 Irapuato, GTO, Mexico

Correspondence
Rafael F. Rivera-Bustamante
rrivera{at}ira.cinvestav.mx

Viral infections usually take place in an orderly manner and can be divided into at least two phases: an early and a late stage. In geminiviruses, plant viruses with a circular, single-stranded DNA genome, expression of viral genes involves complex regulation strategies that suggest the existence of a pattern of temporal gene expression. In this work, the transcription of pepper huasteco yellow vein virus (PHYVV) genes was studied. Green fluorescent protein replacements and RT-PCR analyses were used to monitor PHYVV gene expression chronologically in suspension cells and plant tissue. A model is proposed to describe the order of geminivirus gene expression, where the genes that encode Rep, TrAP and REn are expressed during an early stage of infection. The genes that encode the coat protein and the nuclear shuttle protein are expressed during the late stage of infection.

A supplementary table showing primers used in this study and a supplementary figure showing relative RNA quantification of leftward transcripts are available with the online version of this paper.