HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Appendices A-C Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Arnold, M. E. Articles by Wells, G. A. H. Search for Related Content PubMed PubMed Citation Articles by Arnold, M. E. Articles by Wells, G. A. H. Agricola Articles by Arnold, M. E. Articles by Wells, G. A. H.

Estimating the temporal relationship between PrP^Sc detection and incubation period in experimental bovine spongiform encephalopathy of cattle

S. Czub^2,

A. Davis^1,

A. R. Austin^1,

¹ Veterinary Laboratories Agency (VLA), Woodham Lane, New Haw, Addlestone, Surrey KT15 3NB, UK
² Canadian Food Inspection Agency, Winnipeg Laboratory, Winnipeg, MB R3E 3M4, Canada
³ Health Canada, Health Products and Food Branch, Tunney's Pasture, Ottawa, ON K1A 0K9, Canada

Correspondence
M. E. Arnold
m.arnold{at}vla.defra.gsi.gov.uk

This study examines tissues from sequential-kill, time-course pathogenesis studies to refine estimates of the age at which disease-specific PrP (PrP^Sc) can first be detected in the central nervous system (CNS) and related peripheral nervous system ganglia of cattle incubating bovine spongiform encephalopathy (BSE). Such estimates are important for risk assessments of the age at which these tissues should be removed from cattle at slaughter to prevent human and animal exposure to BSE infection. Tissues were examined from cattle dosed orally with 100 or 1 g BSE-infected brain. Incubation period data for the doses were obtained from attack rate and the sequential-kill studies. A statistical model, fitted by maximum likelihood, accounted for the differences in the lognormal incubation period and the logistic probability of infection between different dose groups. Initial detection of PrP^Sc during incubation was invariably in the brainstem and the earliest was at 30 and 44 months post-exposure for the 100 g- and 1 g-dosed sequential-kill study groups, respectively. The point at which PrP^Sc in 50 % of the animals would be detected by immunohistochemistry applied to medulla–obex was estimated at 9.6 and 1.7 months before clinical onset for the 100 g- and 1 g-dosed cattle, respectively, with a low probability of detection in any of the tissues examined at more than 12 months before clinical onset. PrP^Sc was detected inconsistently in dorsal root ganglia, concurrent with or after detection in CNS, and not at all in certain sympathetic nervous system ganglia.

Present address: National and OIE BSE Reference Laboratories, Animal Diseases Research Institute, Canadian Food Inspection Agency, Township Road 9-1, Lethbridge, AB T1J 3Z4, Canada.

Present address: Pathology, Infectious Disease and Biosecurity, School of Veterinary Science, University of Queensland, St Lucia, QLD 4072, Australia.

Present address: Oak Farm, Harpsden Bottom, Henley-on-Thames, Oxon RG9 4HY, UK.

Appendices A (including Supplementary Table S1 and Supplementary Fig. S1), B and C are available with the online version of this paper.

This article has been cited by other articles:

				H. J. Sohn, Y. H. Lee, R. B. Green, Y. I. Spencer, S. A. C. Hawkins, M. J. Stack, T. Konold, G. A. H. Wells, D. Matthews, I. S. Cho, et al. Bone marrow infectivity in cattle exposed to the bovine spongiform encephalopathy agent Vet Rec., February 28, 2009; 164(9): 272 - 273. [Full Text] [PDF]

				Y. H. Lee, M. M. Simmons, S. A. C. Hawkins, Y. I. Spencer, P. Webb, M. J. Stack, and G. A. H. Wells Detection of Pathologic Prion Protein in the Olfactory Bulb of Natural and Experimental Bovine Spongiform Encephalopathy Affected Cattle in Great Britain Vet. Pathol., January 1, 2009; 46(1): 59 - 62. [Abstract] [Full Text] [PDF]