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J Gen Virol 88 (2007), 3341-3346; DOI 10.1099/vir.0.83198-0

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Short Communication

Expression of GB virus C NS5A protein from genotypes 1, 2, 3 and 5 and a 30 aa NS5A fragment inhibit human immunodeficiency virus type 1 replication in a CD4+ T-lymphocyte cell line

Qing Chang1, James H. McLinden1, Jack T. Stapleton1, M. Aslam Sathar2 and Jinhua Xiang1

1 Iowa City VA Medical Center and the University of Iowa, Iowa City, IA 52246, USA
2 Nelson R. Mandela School of Medicine, University of Kwazulu-Natal, South Africa

Correspondence
Jinhua Xiang
jinhua-xiang{at}uiowa.edu

GB virus type C (GBV-C) is a common human flavivirus that has been associated with prolonged survival in HIV-positive individuals in several, though not all, epidemiological studies. There are five distinct GBV-C genotypes that are geographically localized, and it has been speculated that GBV-C genotypic differences may explain variable outcomes observed in different clinical studies. Expression of an 85 aa fragment of the GBV-C NS5A phosphoprotein (genotype 2) in a CD4+ T cell line (Jurkat) resulted in inhibition of HIV replication, mediated in part by decreased surface expression of the HIV coreceptor CXCR4 and upregulation of SDF-1. We expressed the NS5A protein from genotypes 1, 2, 3 and 5 in Jurkat cells, and demonstrated that all genotypes inhibited HIV replication. Further deletion mapping demonstrated that expression of a 30 aa fragment resulted in decreased CXCR4 surface expression, upregulation of SDF-1 and inhibition of HIV replication.






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