HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Supplementary Material Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Maori, E. Articles by Sela, I. Search for Related Content PubMed PubMed Citation Articles by Maori, E. Articles by Sela, I. Agricola Articles by Maori, E. Articles by Sela, I.

Isolation and characterization of Israeli acute paralysis virus, a dicistrovirus affecting honeybees in Israel: evidence for diversity due to intra- and inter-species recombination

The Hebrew University of Jerusalem, Faculty of Agricultural, Food and Environmental Quality Sciences, Rehovot 76100, Israel

Correspondence
Ilan Sela
sela{at}agri.huji.ac.il

We report the isolation, purification, genome-sequencing and characterization of a picorna-like virus from dead bees in Israel. Sequence analysis indicated that IAPV (Israeli acute paralysis virus) is a distinct dicistrovirus. It is most homologous to Kashmir bee virus and acute bee paralysis virus. The virus carries a 9487 nt RNA genome in positive orientation, with two open reading frames separated by an intergenic region, and its coat comprises four major proteins, the sizes of which suggest alternate processing of the polyprotein. IAPV virions also carry shorter, defective-interfering (DI)-like RNAs. Some of these RNAs are recombinants of different segments of IAPV RNA, some are recombinants of IAPV RNA and RNA from another dicistrovirus, and yet others are recombinants of IAPV and non-viral RNAs. In several of the DI-like RNAs, a sense-oriented fragment has recombined with its complement, forming hairpins and stem–loop structures. In previous reports, we have shown that potyviral and IAPV sequences are integrated into the genome of their respective hosts. The dynamics of information exchange between virus and host and the possible resistance-engendering mechanisms are discussed.

The GenBank/EMBL/DDBJ accession number of the IAPV sequence reported in this paper is EF219380.

Supplementary material is available with the online version of this paper.

This article has been cited by other articles:

				G. Palacios, J. Hui, P. L. Quan, A. Kalkstein, K. S. Honkavuori, A. V. Bussetti, S. Conlan, J. Evans, Y. P. Chen, D. vanEngelsdorp, et al. Genetic Analysis of Israel Acute Paralysis Virus: Distinct Clusters Are Circulating in the United States J. Virol., July 1, 2008; 82(13): 6209 - 6217. [Abstract] [Full Text] [PDF]