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J Gen Virol 88 (2007), 991-997; DOI 10.1099/vir.0.82673-0

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Phylogenetic analysis of Heliothis armigera cytoplasmic polyhedrosis virus type 14 and a series of dwarf segments found in the genome

Yanqiu Li, Jiamin Zhang, Yang Li, Li Tan, Wuguo Chen, Haishan Luo and Yuanyang Hu

Laboratory of Insect Virology, State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan 430072, Hubei, China

Correspondence
Yuanyang Hu
yyhu{at}whu.edu.cn

Full-length nucleotide sequences for the genome segments (S1–S6) of Heliothis armigera cytoplasmic polyhedrosis virus type 14 (HaCPV-14) have been characterized. Each segment consists of a single open reading frame with conserved motifs AGAA and AGCU at the 5' and 3' ends, respectively. Comparison of the proteins of HaCPV-14 with those of other members of the family Reoviridae suggests that S1 encodes an RNA-dependent RNA polymerase (RdRp), whilst S2 encodes a major capsid protein of the virus. Phylogenetic analysis of RdRps from 16 viruses in the family Reoviridae reveals that the genera Cypovirus and Oryzavirus may have originated from a common insect virus ancestor. A series of viable dwarf segments originating from S5 of HaCPV-14 has been identified. Analysis of the predicted secondary structures for these dwarf segments suggests that the signals essential for replication and packaging are located within the terminal sequences of these segments.

The GenBank/EMBL/DDBJ accession numbers for segments S1–S6 of HaCPV-14 are DQ242048, DQ388474–DQ388477 and DQ017080, respectively, and those for the dwarf segments are DQ677368–DQ677371.

A supplementary table showing primers used for amplification of 5' and 3' parts of segments S1–S6 of the HaCPV genome is available in JGV Online.






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