J Gen Virol Try Microbiology Online
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Gen Virol 88 (2007), 1288-1294; DOI 10.1099/vir.0.82330-0

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Venturi, G.
Right arrow Articles by Ciufolini, M. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Venturi, G.
Right arrow Articles by Ciufolini, M. G.
Agricola
Right arrow Articles by Venturi, G.
Right arrow Articles by Ciufolini, M. G.

Genetic variability of the M genome segment of clinical and environmental Toscana virus strains

Giulietta Venturi1, Massimo Ciccozzi1, Stefania Montieri1, Alessandro Bartoloni2, Daniela Francisci3, Loredana Nicoletti1, Claudia Fortuna1, Luigi Marongiu1, Giovanni Rezza1 and Maria Grazia Ciufolini1

1 Department of Infectious, Parasitic and Immune-mediated Diseases, Istituto Superiore di Sanità, Viale Regina Elena 299, Rome 00161, Italy
2 Division of Infectious and Tropical Diseases, University of Florence, Italy
3 Division of Infectious Diseases, Department of Experimental Medicine and Biochemical Sciences, University of Perugia, Italy

Correspondence
Maria Grazia Ciufolini
mariagrazia.ciufolini{at}iss.it

Twenty-seven strains of Toscana virus, collected over a period of 23 years and isolated from several localities and from different hosts (humans, arthropods and a bat), were investigated by sequencing of a portion of the M genomic segment comprising the GN glycoprotein coding region. Sequence data indicated that the divergence among isolates ranged from 0 to 5.7 % at the nucleotide level and from 0 to 3.4 % at the amino acid level. Phylogenetic analysis revealed four main clusters. A close correspondence between viral strains and area/year of isolation could not be demonstrated, whilst co-circulation of different viral strains in the same area and in the same time period was observed for both patients and environmental viral isolates. Alignment of the deduced amino acid sequences and evolutionary analysis indicated that most of the sites along the gene may be invariable because of purifying and/or neutral selection.

The GenBank/EMBL/DDBJ accession numbers for the nucleotide sequences of the TosV M segment are DQ479890–DQ479916.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 2007 by the Society for General Microbiology.