HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Supplementary material Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Sawiris, G. P. Articles by Rohwer, R. G. Search for Related Content PubMed PubMed Citation Articles by Sawiris, G. P. Articles by Rohwer, R. G. Agricola Articles by Sawiris, G. P. Articles by Rohwer, R. G.

Molecular analysis of bovine spongiform encephalopathy infection by cDNA arrays

¹ Research Service, VA Maryland Healthcare System, Baltimore, MD, USA
² Gene Expression and Genomics Unit, National Institute on Aging, Baltimore, MD, USA
³ University of Maryland School of Medicine, Department of Neurology, Baltimore, MD, USA

Correspondence
Robert G. Rohwer
rrohwer{at}umaryland.edu

Here, the first cDNA array analysis of differential gene expression in bovine spongiform encephalopathy (BSE) is reported, using a spotted cDNA array platform representing nearly 17 000 mouse genes. Array analysis identified 296 gene candidates for differential expression in brain tissue from VM mice in late-stage infection with the 301V strain of BSE, compared with brain tissue from normal, age-matched VM mice. Real-time PCR confirmed differential expression of 25 of 31 genes analysed. Some of the genes identified by array analysis as being expressed differentially are associated with ubiquitin/proteasome function, lysosomal function, molecular chaperoning of protein folding or apoptosis. Other genes are involved in calcium ion binding/homeostasis, zinc ion binding/homeostasis or regulation of transcription. Principal-component analysis shows that the global gene-expression profiles of the BSE-infected samples have gene-expression signatures that are markedly different from, and completely non-overlapping with, those obtained from the normal controls.

A supplementary figure showing replicate analysis of hybridization results and supplementary tables showing primer information and named genes identified by cDNA array as being expressed differentially in BSE-infected mouse brain are available in JGV Online.

This article has been cited by other articles:

				Y. Tang, W. Xiang, S. A. C. Hawkins, H. A. Kretzschmar, and O. Windl Transcriptional Changes in the Brains of Cattle Orally Infected with the Bovine Spongiform Encephalopathy Agent Precede Detection of Infectivity J. Virol., September 15, 2009; 83(18): 9464 - 9473. [Abstract] [Full Text] [PDF]