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Infectivity, pseudorecombination and mutagenesis of Kenyan cassava mosaic begomoviruses

Simon E. Bull^1,

Rob W. Briddon^1,

William S. Sserubombwe^1,

¹ Department of Disease and Stress Biology, John Innes Centre (JIC), Colney Lane, Norwich NR4 7UH, UK
² Kenya Agricultural Research Institute, Katumani Applied Biotechnology Laboratory, PO Box 340, Machakos, Kenya

Correspondence
Rob W. Briddon
robbriddon{at}nibge.org

Cloned DNA-A and DNA-B components of Kenyan isolates of East African cassava mosaic virus (EACMV, EACMV-UG and EACMV-KE2), East African cassava mosaic Kenya virus (EACMKV) and East African cassava mosaic Zanzibar virus (EACMZV) are shown to be infectious in cassava. EACMV and EACMKV genomic components have the same iteron sequence (GGGGG) and can form viable pseudorecombinants, while EACMZV components have a different sequence (GGAGA) and are incompatible with EACMV and EACMKV. Mutagenesis of EACMZV has demonstrated that open reading frames (ORFs) AV1 (encoding the coat protein), AV2 and AC4 are not essential for a symptomatic infection of cassava, although mutants of both ORF AV1 and AV2 produce attenuated symptoms in this host. Furthermore, ORF AV1 and AV2 mutants were compromised for coat protein production, suggesting a close structural and/or functional relationship between these coding regions or their protein products.

Deceased 30 July 2004.

Present address: Department of Biology and Biochemistry, University of Bath, Claverton Down, Bath, Avon BA2 7AY, UK.

Present address: Plant Biotechnology Division, National Institute for Biotechnology and Genetic Engineering, Jhang Road, Faisalabad, Pakistan.

Sequences of primers used are available as supplementary material in JGV Online.

This article has been cited by other articles:

				W. S. Sserubombwe, R. W. Briddon, Y. K. Baguma, G. N. Ssemakula, S. E. Bull, A. Bua, T. Alicai, C. Omongo, G. W. Otim-Nape, and J. Stanley Diversity of begomoviruses associated with mosaic disease of cultivated cassava (Manihot esculenta Crantz) and its wild relative (Manihot glaziovii Mull. Arg.) in Uganda J. Gen. Virol., July 1, 2008; 89(7): 1759 - 1769. [Abstract] [Full Text] [PDF]