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PrP^CWD in rectal lymphoid tissue of deer (Odocoileus spp.)

¹ Colorado Division of Wildlife, Wildlife Research Center, 317 West Prospect Road, Fort Collins, CO 80526-2097, USA
² Colorado State University Veterinary Diagnostic Laboratory, Colorado State University, Fort Collins, CO 80523, USA
³ Veterinary Laboratories Agency – Lasswade, Pentlands Science Park, Penicuik EH26 0PZ, UK
⁴ Moredun Research Institute, Pentlands Science Park, Penicuik EH26 0PZ, UK
⁵ Department of Molecular Biology, University of Wyoming, 1000 E. University Avenue, Laramie, WY 82071, USA
⁶ Department of Veterinary Sciences, University of Wyoming, 1174 Snowy Range Road, Laramie, WY 82070, USA

Correspondence
Lisa L. Wolfe
lisa.wolfe{at}state.co.us

The utility of rectal lymphoid tissue sampling for the diagnosis of chronic wasting disease (CWD) infections in mule deer (Odocoileus hemionus) and white-tailed deer (Odocoileus virginianus) was evaluated. CWD-associated prion protein (PrP^CWD) deposits were observed in the rectal mucosa from 19 orally inoculated mule deer by 381 days post-inoculation (p.i.); similarly, 45 out of 50 naturally infected mule deer had PrP^CWD in their rectal mucosa. In orally inoculated white-tailed deer, the presence of glycine (G) or serine (S) at codon 96 of the native PrP (denoted 96GG, 96GS or 96SS) appeared to influence the temporal patterns of PrP^CWD deposition: nine out of 11 infected 96GG individuals had PrP^CWD in their rectal mucosa by 342 days p.i., whereas only three out of seven infected 96GS individuals had PrP^CWD in their rectal mucosa by 381 days p.i. and none of three 96SS individuals had PrP^CWD in their rectal mucosa by 751 days p.i. These findings support further evaluation of rectal mucosa sampling in CWD surveillance.

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