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Analysis of two human parvovirus PARV4 genotypes identified in human plasma for fractionation

¹ Division of Virology, National Institute for Biological Standards and Control, Blanche Lane, South Mimms, Potters Bar, Hertfordshire, EN6 3QG, UK
² Blood Systems Research Institute, San Francisco, CA 94118, USA
³ University of California, San Francisco, CA 94118, USA
⁴ Department of Virology, University College London Hospital, The Windeyer Building, 46 Cleveland Street, London W1T 4JF, UK
⁵ Laboratory of Experimental Virology, Department of Medical Microbiology, Center for Infection and Immunity Amsterdam (CINIMA), Academic Medical Center, University of Amsterdam, 1105AZ Amsterdam, The Netherlands
⁶ Laboratory of Immunochemistry, D. I. Ivanovsky Institute of Virology, 123098 Moscow, Russia

Correspondence
Sally A. Baylis
sbaylis{at}nibsc.ac.uk

The presence of the novel parvovirus PARV4 and a related variant, PARV5, was recently demonstrated in pooled plasma used in the manufacture of blood and plasma-derived medicinal products. DNA sequence analysis of nearly full-length genomes of four PARV4 and two PARV5 strains from manufacturing plasma pools is now presented. Like PARV4, PARV5 encodes two non-overlapping open reading frames (ORF1 and ORF2), homologous to the non-structural and capsid proteins of other parvoviruses, respectively. A highly conserved region in ORF2 contains phospholipase A₂ motifs involved in parvovirus infectivity. Hybridization of strand-specific probes to DNA extracted from high-titre, PARV4-positive plasma revealed that the positive and negative strands are packaged into PARV4 virions in similar quantities. This extended analysis of nearly full-length PARV4 and PARV5 sequences suggests that they are closely related genotypes and the use of a single virus name, PARV4, comprising genotypes 1 and 2 (previously termed PARV5) is proposed.

The GenBank/EMBL/DDBJ accession numbers for the PARV4 and PARV5 sequences determined in this study are DQ873386–DQ873391.

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