J Gen Virol Try IJSEM Online
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Gen Virol 88 (2007), 2228-2236; DOI 10.1099/vir.0.82757-0

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Assenberg, R.
Right arrow Articles by Grimes, J. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Assenberg, R.
Right arrow Articles by Grimes, J. M.
Agricola
Right arrow Articles by Assenberg, R.
Right arrow Articles by Grimes, J. M.

Crystal structure of the Murray Valley encephalitis virus NS5 methyltransferase domain in complex with cap analogues

René Assenberg1, Jingshan Ren1,2, Anil Verma1, Thomas S. Walter1, David Alderton1, Robert J. Hurrelbrink3, Stephen D. Fuller2, Stéphane Bressanelli4,5, Raymond J. Owens1, David I. Stuart1,2 and Jonathan M. Grimes1,2

1 Oxford Protein Production Facility, The Henry Wellcome Building for Genomic Medicine, Oxford University, Roosevelt Drive, Oxford OX3 7BN, UK
2 Division of Structural Biology, The Henry Wellcome Building for Genomic Medicine, Oxford University, Roosevelt Drive, Oxford OX3 7BN, UK
3 Department of Virology, Telethon Institute for Child Health Research, University of Western Australia, Perth, WA 6008, Australia
4 CNRS, UMR2472, IFR 115, Virologie Moléculaire et Structurale, 91198 Gif sur Yvette, France
5 INRA, UMR1157, Virologie Moléculaire et Structurale, 91198 Gif sur Yvette, France

Correspondence
Jonathan M. Grimes
jonathan{at}strubi.ox.ac.uk

We have determined the high resolution crystal structure of the methyltransferase domain of the NS5 polypeptide from the Murray Valley encephalitis virus. This domain is unusual in having both the N7 and 2'-O methyltransferase activity required for Cap 1 synthesis. We have also determined structures for complexes of this domain with nucleotides and cap analogues providing information on cap binding, based on which we suggest a model of how the sequential methylation of the N7 and 2'-O groups of the cap may be coordinated.

Coordinates and structure factors are deposited with the Protein Data Bank: MT1, 2px2; MT2, 2px4; MT3, 2px5; MT-GTP, 2px8; MT-GTPG, 2pxa; and MT-GTPA, 2pxc.




This article has been cited by other articles:


Home page
 J. Virol.Home page
B. Zhang, H. Dong, Y. Zhou, and P.-Y. Shi
Genetic Interactions among the West Nile Virus Methyltransferase, the RNA-Dependent RNA Polymerase, and the 5' Stem-Loop of Genomic RNA
J. Virol., July 15, 2008; 82(14): 7047 - 7058.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H. Kroschewski, S. P. Lim, R. E. Butcher, T. L. Yap, J. Lescar, P. J. Wright, S. G. Vasudevan, and A. D. Davidson
Mutagenesis of the Dengue Virus Type 2 NS5 Methyltransferase Domain
J. Biol. Chem., July 11, 2008; 283(28): 19410 - 19421.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
H. Dong, S. Ren, B. Zhang, Y. Zhou, F. Puig-Basagoiti, H. Li, and P.-Y. Shi
West Nile Virus Methyltransferase Catalyzes Two Methylations of the Viral RNA Cap through a Substrate-Repositioning Mechanism
J. Virol., May 1, 2008; 82(9): 4295 - 4307.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 2007 by the Society for General Microbiology.