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J Gen Virol 88 (2007), 2583-2589; DOI 10.1099/vir.0.82967-0

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Establishment of productively infected walleye dermal sarcoma explant cells

Joel Rovnak1, Rufina N. Casey2, Connie D. Brewster1, James W. Casey2 and Sandra L. Quackenbush1

1 Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO, USA
2 Department Microbiology and Immunology, Cornell University, Ithaca, NY, USA

Correspondence
Joel Rovnak
joel.rovnak{at}colostate.edu

Walleye dermal sarcoma virus (WDSV) is a complex retrovirus associated with dermal sarcomas in walleye fish. Virus expression is tightly regulated and limited to accessory gene transcripts throughout tumour development. During tumour regression, this regulation is lost and the replication of virus is greatly enhanced. Cultured walleye fibroblasts infected in vitro do not produce significant quantities of infectious virus. Tissue culture cells established by explantation of tumour cells were found to harbour WDSV provirus and to express accessory and structural proteins. The sequence of the provirus showed little variation from a previous WDSV isolate. Retroviral particles were isolated from supernatants from these cells and were able to transfer infection to uninfected walleye fibroblasts. In addition to the virus present in supernatants, much of the virus was cell associated and liberated only by sonication. This virus was found at internal cellular membranes, including mitochondria, and was infectious.

The GenBank/EMBL/DDBJ accession number of the sequence determined in this paper is EF428979.






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