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J Gen Virol 88 (2007), 2605-2610; DOI 10.1099/vir.0.82980-0

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Short Communication

Analysis of the serological variability of Lettuce mosaic virus using monoclonal antibodies and surface plasmon resonance technology

Thierry Candresse1, Hervé Lot2, Sylvie German-Retana1, Renate Krause-Sakate3, John Thomas4, Sylvie Souche2, Thierry Delaunay1, Maryvonne Lanneau1 and Olivier Le Gall1

1 Virologie Végétale, UMR GDPP INRA-Bordeaux 2, IBVM, INRA, BP81, F-33883 Villenave d'Ornon Cedex, France
2 Pathologie Végétale, INRA, BP94, F-84143 Montfavet Cedex, France
3 Departamento de Produção Vegetal, UNESP/FCA, 18603-970 Botucatu, SP, Brazil
4 Department of Primary Industries and Fisheries, Plant Pathology Building, 80 Meiers Road, Indooroopilly, QLD 4068, Australia

Correspondence
Thierry Candresse
tc{at}bordeaux.inra.fr

A panel of 19 monoclonal antibodies (mAbs) was used to study the immunological variability of Lettuce mosaic virus (LMV), a member of the genus Potyvirus, and to perform a first epitope characterization of this virus. Based on their specificity of recognition against a panel of 15 LMV isolates, the mAbs could be clustered in seven reactivity groups. Surface plasmon resonance analysis indicated the presence, on the LMV particles, of at least five independent recognition/binding regions, correlating with the seven mAbs reactivity groups. The results demonstrate that LMV shows significant serological variability and shed light on the LMV epitope structure. The various mAbs should prove a new and efficient tool for LMV diagnostic and field epidemiology studies.

Details of the mAbs used in this study are available as supplementary material with the online version of this paper






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