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J Gen Virol 89 (2008), 130-137; DOI 10.1099/vir.0.83287-0

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Molecular characterization of the first polyomavirus from a New World primate: squirrel monkey polyomavirus

Ernst J. Verschoor1, Marlous J. Groenewoud1, Zahra Fagrouch1, Aruna Kewalapat1, Sabine van Gessel1, Marja J. L. Kik2 and Jonathan L. Heeney1,{dagger}

1 Department of Virology, Biomedical Primate Research Centre, Rijswijk, The Netherlands
2 Department of Pathobiology, Pathology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands

Correspondence
Ernst J. Verschoor
verschoor{at}bprc.nl

DNA samples from a variety of New World monkeys were screened by using a broad-spectrum PCR targeting the VP1 gene of polyomaviruses. This resulted in the characterization of the first polyomavirus from a New World primate. This virus naturally infects squirrel monkeys (Saimiri sp.) and is provisionally named squirrel monkey polyomavirus (SquiPyV). The complete genome of SquiPyV is 5075 bp in length, and encodes the small T and large T antigens and the three structural proteins VP1, VP2 and VP3. Interestingly, the late region also encodes a putative agnoprotein, a feature that it shares with other polyomaviruses from humans, baboons and African green monkeys. Comparison with other polyomaviruses revealed limited sequence similarity to any other polyomavirus, and phylogenetic analysis of the VP1 gene confirmed its uniqueness.

{dagger}Present address: Department of Veterinary Medicine, University of Cambridge, Cambridge, UK.

The GenBank/EMBL/DDBJ accession numbers for the SquiPyV complete genome sequence and partial large T sequences are AM748741 and AM779570–AM779573, respectively.

An alignment of large T gene PCR fragments of SquiPyV and a table showing SquiPyV-specific primers used in this study are available with the online version of this paper.






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