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Pathogenicity of a naturally occurring recombinant DNA satellite associated with tomato yellow leaf curl China virus

Institute of Biotechnology, Zhejiang University, Hangzhou 310029, PR China

Correspondence
Xueping Zhou
zzhou{at}zju.edu.cn

Recombinant DNA β molecules (RecDNA-Aβ) comprising parts of DNA A and DNA β associated with tomato yellow leaf curl China virus (TYLCCNV) have been identified in naturally infected tobacco plants. Several examples of the recombinant DNA have been cloned and characterized by sequence analysis. All are approximately half the size of TYLCCNV genomic DNA, and all contain the βC1 gene and the A-rich region from TYLCCNV DNA β as well as intergenic region sequences and the 5' terminus of the AC1 gene from TYLCCNV DNA A. RecDNA-Aβ was detected by PCR in five of 25 TYLCCNV isolates. Co-inoculation of TYLCCNV DNA A and RecDNA-Aβ induced symptoms indistinguishable from those induced by TYLCCNV DNA A and DNA β in Nicotiana benthamiana, Nicotiana glutinosa, Solanum lycopersicum and Petunia hybrida plants, and Southern blot hybridization results showed that RecDNA-Aβ could replicate stably in N. benthamiana plants.

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