HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Schnettler, E. Articles by Prins, M. Search for Related Content PubMed PubMed Citation Articles by Schnettler, E. Articles by Prins, M. Agricola Articles by Schnettler, E. Articles by Prins, M.

The NS3 protein of rice hoja blanca virus suppresses RNA silencing in mammalian cells

Marcel Prins

Laboratory of Virology, Wageningen University, Binnenhaven 11, 6709 PD Wageningen, The Netherlands

Correspondence
Rob Goldbach
rob.goldbach{at}wur.nl

The NS3 protein of the tenuivirus rice hoja blanca virus (RHBV) has previously been shown to represent the viral RNA interference (RNAi) suppressor and is active in both plant and insect cells by binding short interfering RNAs (siRNAs) in vitro. Using a firefly luciferase-based silencing assay it is described here that NS3 is also active in mammalian cells. This activity is independent of the inducer molecule used. Using either synthetic siRNAs or a short hairpin RNA construct, NS3 was able to significantly suppress the RNAi-mediated silencing of luciferase expression in both monkey (Vero) and human (HEK293) cells. These results support the proposed mode of action of NS3 to act by sequestering siRNAs, the key molecules of the RNAi pathway conserved in all eukaryotes. The possible applications of this protein in modulating RNAi and investigating the proposed antiviral RNAi response in mammalian cell systems are discussed.

Present address: Keygene N.V., Agro Business Park 90, 6708 PW Wageningen, The Netherlands.

This article has been cited by other articles:

				H. Hemmes, L. Kaaij, D. Lohuis, M. Prins, R. Goldbach, and E. Schnettler Binding of small interfering RNA molecules is crucial for RNA interference suppressor activity of rice hoja blanca virus NS3 in plants J. Gen. Virol., July 1, 2009; 90(7): 1762 - 1766. [Abstract] [Full Text] [PDF]