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Molecular characterization of a novel adult diarrhoea rotavirus strain J19 isolated in China and its significance for the evolution and origin of group B rotaviruses

¹ Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou city, Hainan province 571737, PR China
² State Key Laboratory for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, PR China

Correspondence
Shaozhong Ji
szji002{at}yahoo.com.cn

The complete genome of a novel adult diarrhoea rotavirus strain J19 was cloned and sequenced using an improved single-primer sequence-independent method. The complete genome is 17 961 bp and is AU-rich (66.49 %). Northern blot analysis and genomic sequence analysis indicated that segments 1–11 encode 11 viral proteins, respectively. Protein alignments with the corresponding proteins of J19 with B219, and groups A, B and C rotaviruses, produced higher per cent sequence identities to B219. Among groups A, B and C rotaviruses, 10 proteins from group B rotaviruses exhibited slightly higher amino acid sequence identity to the J19 proteins, but proteins of J19 showed low amino acid sequence identity with groups A and C rotaviruses. Construction of unrooted phylogenetic trees using a set of known proteins and representatives of three known rotavirus groups revealed that six structural proteins were positioned close to B219 and the basal nodes of groups A, B and C lineages, although with a preferred association with group B lineages. Phylogenetic analysis of the five non-structural proteins showed a similar trend. The results of the serological analysis, protein sequence analysis and phylogenetic analysis suggested that J19 would be a novel rotavirus strain with great significance to the evolution and origin of group B rotaviruses.

The GenBank/EMBL/DDBJ accession numbers for the full genomic sequences and their deduced protein sequence data of the novel adult diarrhoea rotavirus strain J19 are: VP1 gene DQ113897, protein AAZ03485.1; VP2 gene DQ113898, protein AAZ03486.1; VP3 gene DQ113900, protein AAZ03488.1; VP4 gene DQ113899, protein AAZ03487.1; VP6 gene DQ113902, protein AAZ03490.1; VP7 gene DQ113905, protein AAZ03493.1; NSP1 gene DQ113901, protein AAZ03489.1; NSP2 gene DQ113903, protein AAZ03491.1; NSP3 gene DQ113904, protein AAZ03492.1; NSP4 gene DQ113906, protein AAZ03494.1; NSP5 gene DQ113907, protein AAZ03495.1.

Supplementary material is available with the online version of this paper.

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