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J Gen Virol 89 (2008), 2799-2805; DOI 10.1099/vir.0.2008/003855-0

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Short Communication

Naturally occurring feline leukemia virus subgroup A and B infections in urban domestic cats

Fabiana Magalhães Coelho1, Maria Rosa Quaresma Bomfim2, Fabíola de Andrade Caxito1, Natália Almeida Ribeiro1, Marcela Miranda Luppi1, Érica Azevedo Costa1, Maria Emilia Oliveira1, Flávio Guimarães Da Fonseca1 and Mauricio Resende1

1 Laboratório de Virologia Comparada, Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Avenida Antônio Carlos 6627, CEP: 31270-901, Belo Horizonte, MG, Brazil
2 Laboratório de Vírus, Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Avenida Antônio Carlos 6627, CEP: 31270-901, Belo Horizonte, MG, Brazil

Correspondence
Fabiana Magalhães Coelho
fabismc{at}yahoo.com.br

A nested-PCR (n-PCR) was used to detect feline leukemia virus (FeLV) proviral DNA in blood samples from 464 sick and 608 healthy domestic cats (Felis catus) selected by convenience, and a significantly high prevalence of FeLV infection was observed. n-PCR results revealed the presence of FeLV proviral DNA in 47.2 % of sick cats and 47.4 % of healthy cats. Phylogenetic analysis revealed that FeLV samples from healthy or sick cats were grouped into separate clades. We determined FeLV subgroups by an n-PCR based on the envelope (env) gene. The partial env gene of FeLV Minas Gerais (MG) samples were compared to various exogenous FeLV isolates and endogenous (enFeLV) provirus from the same region. FeLV-B MG samples were more similar to endogenous sequences and to natural FeLV-B isolates than to either FeLV-A or FeLV-C. The results revealed the circulation of FeLV-B in large populations of urban domestic cats in Brazil.

The GenBank/EMBL/DDBJ accession numbers for the sequences reported in this paper are: AY745878, DQ821499–DQ821502, DQ871351–DQ871353, EU136639, EU436640, EU048344–EU048365, EU048367, EU090943, EU090945–EU090948, EU629217–EU629222, EU783967 and EU636786. Nucleotide sequences corresponding to the env gene of FeLV provirus were also deposited in GenBank/EMBL/DDBJ [accession numbers: 843B-MG (EU783973), 887-MG (EU783967), 914B-MG (EU636786), 918B-MG (EU629220), 922B-MG (EU629222), 1230B-MG (EU629221), 328A-MG (EU629217), 1235A-MG (EU629218) and 1286A-MG (EU629219)].






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