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Transcripts encoding the nanovirus master replication initiator proteins are terminally redundant

Institut des Sciences du Végétal, CNRS, 91198 Gif sur Yvette, France

Correspondence
Bruno Gronenborn
gronenborn{at}isv.cnrs-gif.fr

The multicomponent single-stranded DNA plant nanoviruses encode unique master replication initiator (Rep) proteins. We have mapped the 5' and 3' termini of the corresponding polyadenylated mRNAs from faba bean necrotic yellows virus (FBNYV) and subterranean clover stunt virus and found that these are terminally redundant by up to about 160 nt. Moreover, the origin of viral DNA replication is transcribed into RNA that is capable of folding into extended secondary structures. Other nanovirus genome components, such as the FBNYV DNA encoding the protein Clink or an FBNYV DNA encoding a non-essential para-Rep protein, are not transcribed in such a unique fashion. Thus, terminally redundant mRNAs and the resulting transcription of the replication origin appear to be restricted to nanovirus master Rep DNAs. We speculate that this may be a way to regulate the expression of the essential master Rep protein.

A supplementary figure showing transcripts of FBNYV DNA-R, SCSV DNA-R, FBNYV C11 and FBNYV DNA-C and a supplementary table showing oligonucleotides used in this study are available with the online version of this paper.