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Identification of two distinct bovine parainfluenza virus type 3 genotypes

Department of Primary Industries and Fisheries, Ritchie Building 64A, Level 3, C Wing, Research Road, St Lucia, QLD 4072, Australia

Correspondence
Paul Francis Horwood
paul.horwood{at}dpi.qld.gov.au

The partial gene sequencing of the matrix (M) protein from seven clinical isolates of bovine parainfluenza virus type 3 (BPIV-3), and the complete sequencing of a representative isolate (Q5592) was completed in this study. Nucleotide sequence analysis was initiated because of the failure of in-house BPIV-3 RT-PCR methods to yield expected products for four of the isolates. Phylogenetic reconstructions based on the nucleotide sequences for the M-protein and the entire genome, using all of the available BPIV-3 nucleotide sequences, demonstrated that there were two distinct BPIV-3 genotypes (BPIV-3a and BPIV-3b). These newly identified genotypes have implications for the development of BPIV-3 molecular detection methods and may also impact on BPIV-3 vaccine formulations.

The GenBank/EMBL/DDBJ accession numbers for the sequences reported in this paper are EF108221–EF108225, EU266069 and EU277658.

Supplementary figures are available with the online version of this paper.