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Immunolocalization of deformed wing virus particles within the mite Varroa destructor

Ma. Teresa Santillán-Galicia^1,2,

Raffaella Carzaniga^3,

Brenda V. Ball^1,

¹ Centre for Soils and Ecosystem Function, Department of Plant and Invertebrate Ecology, Rothamsted Research, Harpenden AL5 2JQ, UK
² School of Biosciences, The University of Nottingham, Sutton Bonington Campus, Loughborough LE12 5RD, UK
³ Centre for Bioimaging, Department of Plant Pathology and Microbiology, Rothamsted Research, Harpenden AL5 2JQ, UK

Correspondence
Ma. Teresa Santillán-Galicia
tgalicia{at}colpos.mx

Deformed wing virus (DWV) induces wing deformation when bees are infected during their pupal development. Field observations and laboratory experiments suggest that the mite Varroa destructor is a vector of the virus. Moreover, it has been stated that DWV replicates within this mite. In order to understand the role of V. destructor in the transmission of DWV, the objective of this work was to locate the sites of retention and/or replication of DWV within the mite by immunohistochemistry. There was no evidence that DWV was replicating in the mite as no tissues showed specific antibody binding to DWV. Also, there were no specific structures that could be suggested as retention sites. DWV was found only in the midgut lumen of V. destructor in structures resembling large, dense spheres, which were presumably faecal pellets.

Present address: Colegio de Postgraduados, Postgrado en Entomología y Acarología, Carretera Federal México-Texcoco, Km. 36.5 Montecillo, Texcoco, CP 56230, Estado de México, Mexico.

Present address: Centre for Molecular Microbiology and Infection, Department of Infectious Diseases, 5th floor Wolfson Building, South Kensington Campus, Imperial College London, London SW7 2AZ, UK.

Present address: 104 lower Luton Road, Wheathampstead, St Albans AL4 8HH, UK.

||Present address: School of Biosciences, The University of Nottingham, Malaysia Campus, Jalan Broga, 43500 Semenyih, Selangor Darul Ehsan, Malaysia.