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J Gen Virol 9 (1970), 1-7; DOI 10.1099/0022-1317-9-1-1
© 1970 Society for General Microbiology
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The Effect of Growth Rate in Continuous-flow Cultures on the Replication of Rubella Virus in BHK Cells

D. G. Kilburn* and A. L. Van Wezel

National Institute for Public Health, Bilthoven, The Netherlands

Baby hamster kidney cells infected with rubella virus were grown in continuous flow culture to investigate the effect of growth rate on virus replication. Both cell and virus concentration varied with growth rate in normal Eagle's medium but were independent of growth rate in phosphate-limited medium. In both media, virus production rate increased with increasing growth rate but there was a concomitant increase in the fraction of the cell population that was shedding virus, so it is not known if there was a significant increase in the synthetic rate at the cellular level. A sharp decline in virus titre followed each change in dilution rate or the onset of phosphate limitation. From the kinetics of the decline it appears that the apparatus for virus synthesis was not renewed during the 40 to 60 hr following perturbation; existing synthetic apparatus washed out exponentially without detectable decay. In continuous flow cultures, growth could be maintained for months without reduction in virus titre, complement-fixing antigen or antibody-inducing capacity.

* Present address: Department of Microbiology, University of British Columbia, Vancouver 8, B.C., Canada.

Received 6 May 1970; accepted 20 May 1970.




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Copyright © 1970 by the Society for General Microbiology.