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Originally published as JGV in Press, 10.1099/vir.0.013250-0 on June 24, 2009 J Gen Virol 90 (2009), 2375-2380; DOI 10.1099/vir.0.013250-0

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Short Communication

High-throughput sequence analysis of variants of human cytomegalovirus strains Towne and AD169

Amanda J. Bradley1,{dagger}, Nell S. Lurain2, Peter Ghazal3, Urmi Trivedi4, Charles Cunningham1, Katarina Baluchova1, Derek Gatherer1, Gavin W. G. Wilkinson5, Derrick J. Dargan1 and Andrew J. Davison1

1 MRC Virology Unit, Institute of Virology, Church Street, Glasgow G11 5JR, UK
2 Department of Immunology and Microbiology, Rush University Medical Center, 1653 West Congress Parkway, Chicago, IL 60612, USA
3 Division of Pathway Medicine, University of Edinburgh Medical School, Chancellor's Building, 49 Little France Crescent, Edinburgh EH16 4SB, UK
4 The Gene Pool, Ashworth Laboratories, Institute of Evolutionary Biology, King's Buildings, Edinburgh EH9 3JT, UK
5 Department of Medical Microbiology, Tenovus Building, School of Medicine, Cardiff University, Heath Park, Cardiff CF14 4XX, UK

Correspondence
Andrew J. Davison
a.davison{at}mrcvu.gla.ac.uk

The genomes of commonly used variants of human cytomegalovirus (HCMV) strains Towne and AD169 each contain a substantial mutation in which a region (UL/b') at the right end of the long unique region has been replaced by an inverted duplication of a region from the left end of the genome. Using high-throughput technology, we have sequenced HCMV strain Towne (ATCC VR-977) and confirmed the presence of two variants, one exhibiting the replacement in UL/b' and the other intact in this region. Both variants are mutated in genes RL13, UL1, UL40, UL130, US1 and US9. We have also sequenced a novel AD169 variant (varUC) that is intact in UL/b' except for a small deletion that affects genes UL144, UL142, UL141 and UL140. Like other AD169 variants, varUC is mutated in genes RL5A, RL13, UL36 and UL131A. A subpopulation of varUC contains an additional deletion affecting genes IRS1, US1 and US2.

{dagger}Present address: BioReliance Ltd, Todd Campus, West of Scotland Science Park, Glasgow G20 0XA, UK.

The GenBank/EMBL/DDBJ accession numbers for the genome sequences of HCMV strains Towne and AD169varUC are FJ616285 [GenBank] and FJ527563 [GenBank] , respectively.

The Illumina Genome Analyzer sequence datasets are available from the corresponding author.






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