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Originally published as JGV in Press, 10.1099/vir.0.008474-0 on July 8, 2009 J Gen Virol 90 (2009), 2604-2608; DOI 10.1099/vir.0.008474-0

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Short Communication

Smallpox vaccines induce antibodies to the immunomodulatory, secreted vaccinia virus complement control protein

Joan E. Adamo, Clement A. Meseda, Jerry P. Weir and Michael J. Merchlinsky

Laboratory of DNA Viruses, Center for Biologics Evaluation and Research (CBER), US Food and Drug Administration (FDA), Bethesda, MD 20892, USA

Correspondence
Michael J. Merchlinsky
michael.merchlinsky{at}hhs.gov

Vaccination with Dryvax elicits a broad humoral response against many viral proteins. Human vaccinia immune globulin was used to screen the secreted proteins from cells infected with Dryvax or the candidate smallpox vaccine LC16m8 to determine whether the protective humoral response included antibodies against secreted viral proteins. Many proteins were detected, with the primary band corresponding to a band of 28 or 30 kDa in cells infected with Dryvax or LC16m8, respectively. This was identified as the vaccinia virus complement protein (VCP), which migrated more slowly in LC16m8-infected cells due to post-translational glycosylation. Vaccinia virus deleted in VCP, vVCPko, protected mice from a lethal intranasal challenge of vaccinia Western Reserve strain. Mice vaccinated with purified VCP demonstrated a strong humoral response, but were not protected against a moderate lethal challenge of vaccinia virus, suggesting that the humoral response against VCP is not critical for protection.






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