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Originally published as JGV in Press, 10.1099/vir.0.014118-0 on July 29, 2009 J Gen Virol 90 (2009), 2746-2750; DOI 10.1099/vir.0.014118-0

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Short Communication

Virulence of pigeon paramyxovirus type 1 does not always correlate with the cleavability of its fusion protein

J. C. F. M. Dortmans1,2, G. Koch1, P. J. M. Rottier2 and B. P. H. Peeters1

1 Central Veterinary Institute (CVI) of Wageningen UR, Lelystad, The Netherlands
2 Virology Division, Department of Infectious Diseases and Immunology, Utrecht University, Faculty of Veterinary Medicine, Utrecht, The Netherlands

Correspondence
B. P. H. Peeters
Ben.Peeters{at}wur.nl

Some pigeon paramyxovirus type 1 (PPMV-1) strains exhibit low virulence in chickens, despite their fusion (F) protein's multi-basic cleavage site. To elucidate the molecular basis of the low pathogenicity of these strains, we constructed an infectious full-length cDNA clone of PPMV-1 strain AV324. This strain is non-virulent for chickens, although its F protein contains the typical virulence motif 112RRKKRF117. By using reverse genetics, we exchanged the F genes of AV324 and a virulent Newcastle disease virus (NDV) strain (Herts) and evaluated the recovered chimeric viruses for their pathogenicity in 1-day-old chickens and in embryonated eggs. Our results show that the F protein of AV324, and probably those of similar PPMV-1 strains, are functionally not different from those of virulent NDV strains and that the difference in pathogenicity must be determined by other factors.

The GenBank/EMBL/DDBJ accession number for the sequence of AV324 is GQ429292 [GenBank] and that for Dove/Italy/2736/00 is GQ429293.






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