J Gen Virol
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published as JGV in Press, 10.1099/vir.0.013912-0 on August 26, 2009 J Gen Virol 90 (2009), 2940-2951; DOI 10.1099/vir.0.013912-0

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplementary Table
Right arrow All Versions of this Article:
vir.0.013912-0v1
90/12/2940    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via CrossRef
Google Scholar
Right arrow Articles by Olveira, J. G.
Right arrow Articles by Bandín, I.
PubMed
Right arrow PubMed Citation
Right arrow Articles by Olveira, J. G.
Right arrow Articles by Bandín, I.
Agricola
Right arrow Articles by Olveira, J. G.
Right arrow Articles by Bandín, I.

Comparative analysis of both genomic segments of betanodaviruses isolated from epizootic outbreaks in farmed fish species provides evidence for genetic reassortment

J. G. Olveira1, S. Souto1, C. P. Dopazo1, R. Thiéry2, J. L. Barja1 and I. Bandín1

1 Unidad de Ictiopatología-Patología Viral, Departamento de Microbiología y Parasitología, Instituto de Acuicultura, Universidad de Santiago de Compostela, Spain
2 French Food Safety Agency, BP111, F-06902 Sophia Antipolis, France

Correspondence
Isabel Bandín
isabel.bandin{at}usc.es

Sequencing of the full coding region of both genomic segments of seven betanodavirus strains isolated from different farmed species in Spain and Portugal revealed that six were reassortants, exhibiting a red-spotted grouper nervous necrosis virus (RGNNV)-type RNA1 and a striped jack nervous necrosis virus (SJNNV)-type RNA2. Analysis of sequences of reassortant strains at both the genomic and protein levels revealed the existence of differences compared with type strains of both genotypes. These differences were greater in the polymerase sequence, which is remarkable because viral structural proteins generally diverge more rapidly than non-structural proteins. Changes in two amino acids observed in the SJNNV capsid protein might be involved in the colonization of new host species by these reassortant strains. In addition, a more extensive phylogenetic analysis, including partial sequences of both RNA segments of 16 other Iberian nodaviruses, confirmed the existence of reassortment between RGNNV and SJNNV.

The GenBank/EMBL/DDBJ accession numbers for the sequences reported in this paper are FJ803911 [GenBank] –FJ803923 [GenBank] , FJ829452 [GenBank] and GQ131547 [GenBank] –GQ131571.

A table of primer sequences used for sequencing is available with the online version of this paper.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 2009 by the Society for General Microbiology.