HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Supplementary tables and figure All Versions of this Article: vir.0.012112-0v1 90/12/3042    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Google Scholar Articles by Zhang, X. Articles by Wong, S.-M. PubMed PubMed Citation Articles by Zhang, X. Articles by Wong, S.-M. Agricola Articles by Zhang, X. Articles by Wong, S.-M.

Hibiscus chlorotic ringspot virus upregulates plant sulfite oxidase transcripts and increases sulfate levels in kenaf (Hibiscus cannabinus L.)

¹ Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, Kent Ridge, Singapore 117543
² Temasek Life Sciences Laboratory, 1 Research Link, Singapore 117604

Correspondence
Sek-Man Wong
dbswsm{at}nus.edu.sg

Hibiscus chlorotic ringspot virus (HCRSV) coat protein (CP) is required for encapsidation and virus systemic movement. To better understand the roles of HCRSV CP in virus infection and its interactions with host proteins, a cDNA library of kenaf (Hibiscus cannabinus L.) was constructed and screened by using a yeast two-hybrid system (YTHS) to identify CP-interacting proteins. One protein identified was sulfite oxidase (SO) and the interaction was confirmed in vitro and in vivo. The interaction was found to be associated with peroxisomes by immunofluorescent labelling of peroxisomes by an anti-SKL signal peptide antibody. Our YTHS results showed that only the P and S domains of CP interacted with SO from kenaf. This is probably due to the exposure of these two domains on the outer surface of the capsid. Peroxisomes were observed to aggregate in HCRSV-infected cells, and biochemical assays of total protein from kenaf leaf extracts showed that SO activity and SO-dependent H₂O₂-generating activity in the HCRSV-infected leaves increased compared with that in mock-inoculated kenaf plants.

The GenBank/EMBL/DDBJ accession number for the HcSO sequence obtained in this study is FJ603465.

Tables showing primers used in this study and putative interacting proteins screened from the kenaf cDNA library, and an amino acid sequence alignment of the sulfite oxidase from kenaf and other plants, are available with the online version of this paper.