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Originally published as JGV in Press, 10.1099/vir.0.014548-0 on September 2, 2009 J Gen Virol 90 (2009), 3051-3056; DOI 10.1099/vir.0.014548-0

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Short Communication

A full-length infectious clone of beet soil-borne virus indicates the dispensability of the RNA-2 for virus survival in planta and symptom expression on Chenopodium quinoa leaves

François Crutzen1, Mohsen Mehrvar1,2, David Gilmer3 and Claude Bragard1

1 Université catholique de Louvain, unité de phytopathologie, Croix du Sud 2 bte 3, B-1348 Louvain-la-Neuve, Belgium
2 Department of Plant Protection, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran
3 Institut de Biologie Moléculaire des Plantes, Laboratoire propre du CNRS (UPR 2357) conventionné avec l'Université de Strasbourg, 12 rue du Général Zimmer, 67084 Strasbourg, France

Correspondence
François Crutzen
francois.crutzen{at}uclouvain.be

For a better understanding of the functionality and pathogenicity of beet soil-borne virus (BSBV), full-length cDNA clones have been constructed for the three genomic RNAs. With the aim of assessing their effectiveness and relative contribution to the virus housekeeping functions, transcripts were inoculated on Chenopodium quinoa and Beta macrocarpa leaves using five genome combinations. Both RNAs-1 (putative replicase) and -3 (putative movement proteins) proved to be essential for virus replication in planta and symptom production on C. quinoa, whereas RNA-2 (putative coat protein, CP, and a read-through domain, RT) was not. No symptoms were recorded on B. macrocarpa, but viral RNAs were detected. In both host plants, the 19 kDa CP was detected by Western blotting as well as a 115 kDa protein corresponding to the CP–RT.

The GenBank/EMBL/DDBJ accession numbers for the sequences reported in this paper are FN386612 [GenBank] , FN386613 [GenBank] and FN386614.






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