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Determination of the henipavirus phosphoprotein gene mRNA editing frequencies and detection of the C, V and W proteins of Nipah virus in virus-infected cells

¹ Measles, Mumps, Rubella and Herpesviruses Laboratory Branch, 1600 Clifton Road, MS-C-22, Atlanta, GA 30333, USA
² Emory University School of Medicine, Department of Microbiology and Immunology, 1510 Clifton Road, Atlanta, GA 30322, USA
³ Commonwealth Scientific Industrial Research Organization, Australian Animal Health Laboratory, 5 Portarlington Road, East Geelong, Victoria, Australia
⁴ The Research Institute at Nationwide Children's Hospital, Center for Vaccines and Immunity, 700 Children's Drive, Columbus, OH 43205, USA
⁵ The Ohio State University, College of Medicine, Department of Pediatrics, Columbus, OH 43205, USA

Correspondence
Paul A. Rota
prota{at}cdc.gov

The henipaviruses, Nipah virus (NiV) and Hendra virus (HeV), are highly pathogenic zoonotic paramyxoviruses. Like many other paramyxoviruses, henipaviruses employ a process of co-transcriptional mRNA editing during transcription of the phosphoprotein (P) gene to generate additional mRNAs encoding the V and W proteins. The C protein is translated from the P mRNA, but in an alternate reading frame. Sequence analysis of multiple, cloned mRNAs showed that the mRNA editing frequencies of the P genes of the henipaviruses are higher than those reported for other paramyxoviruses. Antisera to synthetic peptides from the P, V, W and C proteins of NiV were generated to study their expression in infected cells. All proteins were detected in both infected cells and purified virions. In infected cells, the W protein was detected in the nucleus while P, V and C were found in the cytoplasm.

Three supplementary figures and full materials and methods are available with the online version of this paper.

This article has been cited by other articles:

				S. Kulkarni, V. Volchkova, C. F. Basler, P. Palese, V. E. Volchkov, and M. L. Shaw Nipah Virus Edits Its P Gene at High Frequency To Express the V and W Proteins J. Virol., April 15, 2009; 83(8): 3982 - 3987. [Abstract] [Full Text] [PDF]