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J Gen Virol 90 (2009), 759-763; DOI 10.1099/vir.0.007112-0

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Short Communication

A single U/C nucleotide substitution changing alanine to valine in the beet necrotic yellow vein virus P25 protein promotes increased virus accumulation in roots of mechanically inoculated, partially resistant sugar beet seedlings

R. Koenig1, S. Loss1, J. Specht1, M. Varrelmann2, P. Lüddecke1 and G. Deml1

1 Julius Kühn Institut, Bundesforschungsinstitut für Kulturpflanzen, Institut für Epidemiologie und Pathogendiagnostik, Messeweg 11, D-38106 Braunschweig, Germany
2 Institut für Zuckerrübenforschung, Abteilung Phytomedizin, Holtenser Landstraße 77, D-37079 Göttingen, Germany

Correspondence
R. Koenig
renate.koenig{at}jki.bund.de

Beet necrotic yellow vein virus (BNYVV) A type isolates E12 and S8, originating from areas where resistance-breaking had or had not been observed, respectively, served as starting material for studying the influence of sequence variations in BNYVV RNA 3 on virus accumulation in partially resistant sugar beet varieties. Sub-isolates containing only RNAs 1 and 2 were obtained by serial local lesion passages; biologically active cDNA clones were prepared for RNAs 3 which differed in their coding sequences for P25 aa 67, 68 and 129. Sugar beet seedlings were mechanically inoculated with RNA 1+2/RNA 3 pseudorecombinants. The origin of RNAs 1+2 had little influence on virus accumulation in rootlets. E12 RNA 3 coding for V67C68Y129 P25, however, enabled a much higher virus accumulation than S8 RNA 3 coding for A67H68H129 P25. Mutants revealed that this was due only to the V67 ‘GUU’ codon as opposed to the A67 ‘GCU’ codon.

The GenBank/EMBL/DDBJ accession numbers are EU330453 and EU330450 for RNAs 1, EU330451 and EU330452 for RNAs 2 and EU330455 and EU330454 for RNAs 3 of the E12 and S8 isolates, respectively.






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