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Originally published as JGV in Press, 10.1099/vir.0.008243-0 on March 4, 2009 J Gen Virol 90 (2009), 1014-1024; DOI 10.1099/vir.0.008243-0

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Variability in the level of RNA silencing suppression caused by triple gene block protein 1 (TGBp1) from various potexviruses during infection

Hiroko Senshu1, Johji Ozeki1, Ken Komatsu1, Masayoshi Hashimoto1, Kouji Hatada1, Michiko Aoyama1, Satoshi Kagiwada2, Yasuyuki Yamaji1 and Shigetou Namba1

1 Laboratory of Plant Pathology, Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan
2 Department of Clinical Plant Science, Faculty of Bioscience and Applied Chemistry, Hosei University, Tokyo, Japan

Correspondence
Shigetou Namba
anamba{at}mail.ecc.u-tokyo.ac.jp

RNA silencing is an important defence mechanism against virus infection, and many plant viruses encode RNA silencing suppressors as a counter defence. In this study, we analysed the RNA silencing suppression ability of multiple virus species of the genus Potexvirus. Nicotiana benthamiana plants exhibiting RNA silencing of a green fluorescent protein (GFP) transgene showed reversal of GFP fluorescence when systemically infected with potexviruses. However, the degree of GFP fluorescence varied among potexviruses. Agrobacterium-mediated transient expression assay in N. benthamiana leaves demonstrated that the triple gene block protein 1 (TGBp1) encoded by these potexviruses has drastically different levels of silencing suppressor activity, and these differences were directly related to variations in the silencing suppression ability during virus infection. These results suggest that suppressor activities differ even among homologous proteins encoded by viruses of the same genus, and that TGBp1 contributes to the variation in the level of RNA silencing suppression by potexviruses. Moreover, we investigated the effect of TGBp1 encoded by Plantago asiatica mosaic virus (PlAMV), which exhibited a strong suppressor activity, on the accumulation of microRNA, virus genomic RNA and virus-derived small interfering RNAs.




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A. Kozlowska-Makulska, H. Guilley, M. S. Szyndel, M. Beuve, O. Lemaire, E. Herrbach, and S. Bouzoubaa
P0 proteins of European beet-infecting poleroviruses display variable RNA silencing suppression activity
J. Gen. Virol., April 1, 2010; 91(4): 1082 - 1091.
[Abstract] [Full Text] [PDF]


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