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Originally published as JGV in Press, 10.1099/vir.0.009019-0 on March 4, 2009 J Gen Virol 90 (2009), 1135-1140; DOI 10.1099/vir.0.009019-0

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Short Communication

Expression and processing of human immunodeficiency virus type 1 gp160 using the vesicular stomatitis virus New Jersey serotype vector system

Kunyu Wu, Gyoung Nyoun Kim and C. Yong Kang

Department of Microbiology and Immunology, Schulich School of Medicine and Dentistry, Siebens-Drake Research Institute, The University of Western Ontario, London, ON N6G 2V4, Canada

Correspondence
C. Yong Kang
cykang{at}uwo.ca

The Indiana serotype of vesicular stomatitis virus (VSVIND), but not the New Jersey serotype (VSVNJ), has been widely used as a gene expression vector. In terms of prime–boost-based vaccine strategies, it would be desirable to use two different VSV serotypes to avoid immunity against the priming viral vector. Here, we report that we have applied the VSVNJ vector system for expression of the env gene of human immunodeficiency virus type 1 (HIV-1). The HIV-1 env gene was inserted into the VSVNJ vector system at two different sites: between the P and M genes (NP-gp160-MGL) and between the G and L genes (NPMG-gp160-L). The HIV-1 env gene product, gp160, was efficiently expressed and processed in cells infected with either of these two recombinant VSV–HIV-1gp160 viruses. In this study, we have investigated the applicability of the VSVNJ vector system for foreign gene expression.

Additional methods are available with the online version of this paper.






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