J Gen Virol Email Content Delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published as JGV in Press, 10.1099/vir.0.008227-0 on March 4, 2009 J Gen Virol 90 (2009), 1229-1237; DOI 10.1099/vir.0.008227-0

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplementary Tables
Right arrow All Versions of this Article:
vir.0.008227-0v1
90/5/1229    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wu, E.-q.
Right arrow Articles by Kong, W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wu, E.-q.
Right arrow Articles by Kong, W.
Agricola
Right arrow Articles by Wu, E.-q.
Right arrow Articles by Kong, W.

Profile of physical status and gene variation of human papillomavirus 58 genome in cervical cancer

En-qi Wu1,3,{dagger}, Xiao Zha2,3,{dagger}, Xiang-hui Yu1, Guo-nan Zhang3, Yong-ge Wu1, Ying Fan3, Yuan Ren2, Ling-Qian Kong1 and Wei Kong1

1 College of Life Science, Jilin University, Changchun 130012, PR China
2 Department of Molecular Pharmacology, Sichuan Tumor Hospital, Chengdu 610041, PR China
3 Department of Gynecologic Oncology, Sichuan Tumor Hospital, Chengdu 610041, PR China

Correspondence
Xiao Zha
zha530909{at}sina.com
Wei Kong
weikong{at}mail.jlu.edu.cn

Epidemiological studies have shown that human papillomavirus 58 (HPV 58) is found at a relatively high frequency in east Asia and some regions of Central and South America. To investigate the physical status of HPV 58 and analyse sequence variations of HPV 58 in cervical cancer patients, the HPV 58 genome in 37 HPV 58-positive cervical cancer specimens collected from China were investigated by a mapping analysis based on nested PCR and nucleotide sequencing. A pure integrated genome was found in 78.4 % (29/37) of specimens, which is much higher than that found in previous studies. Multiple disruptions were first found among the integrated HPV 58 genomes in 51.7 % (15/29) of specimens. Among the 7824 bp of the HPV 58 genome, 119 (1.52 %) nucleotide positions were found to be variable, and 45 of them lead to amino acid changes. Phylogenetic analyses, based on partial L1 sequences of 14 variants isolated in previous studies and this study, show that two main groups were observed in HPV 58 variants, the prototype or prototype-like group and the non-prototype-like group.

{dagger}These authors contributed equally to this work.

Supplementary tables are available with the online version of this paper.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 2009 by the Society for General Microbiology.