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Ovine PRNP untranslated region and promoter haplotype diversity

Department of Molecular Pathogenesis and Genetics, Veterinary Laboratories Agency, New Haw, Addlestone, Surrey KT15 3NB, UK

Correspondence
Ginny C. Saunders
g.c.saunders{at}vla.defra.gsi.gov.uk

The diversity and possible contribution of non-coding regions of the prion protein (PrP) gene (PRNP) to transmissible spongiform encephalopathy susceptibility and PrP regulation are not fully known. This study defined ten ovine PRNP promoters and five untranslated region (UTR) haplotypes found in atypical and classical scrapie cases and healthy control sheep. A greater diversity of promoter and UTR haplotypes was observed in conjunction with the ARQ PrP allele (seven promoter and four UTR haplotypes), while it was observed that the other alleles were linked with a limited number of haplotypes, such as ARR, found to be linked to only two promoter and one UTR haplotypes. In silico analysis identified potential transcription factor binding sites that differed in the promoter haplotype variants. Furthermore, a 5' UTR internal ribosome entry site motif was identified in exon 2 and highlights a possible role for this exon in regulating PrP expression at the translational level.

A supplementary figure and supplementary tables of primer sequences and of haplotype variations are available with the online version of this paper.

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				G. C. Saunders, I. Lantier, S. Cawthraw, P. Berthon, S. J. Moore, M. E. Arnold, O. Windl, M. M. Simmons, O. Andreoletti, S. Bellworthy, et al. Protective effect of the T112 PrP variant in sheep challenged with bovine spongiform encephalopathy J. Gen. Virol., October 1, 2009; 90(10): 2569 - 2574. [Abstract] [Full Text] [PDF]