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Originally published as JGV in Press, 10.1099/vir.0.010074-0 on March 12, 2009 J Gen Virol 90 (2009), 1433-1439; DOI 10.1099/vir.0.010074-0

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Genetic and epidemiological characterization of Stretch Lagoon orbivirus, a novel orbivirus isolated from Culex and Aedes mosquitoes in northern Australia

Chris Cowled1, Gustavo Palacios2, Lorna Melville3, Richard Weir3, Susan Walsh3, Steven Davis3, Aneta Gubala1, W. Ian Lipkin2, Thomas Briese2 and David Boyle1

1 CSIRO Livestock Industries, Australian Animal Health Laboratory, East Geelong, VIC 3220, Australia
2 Center for Infection and Immunity, Mailman School of Public Health, Columbia University, New York, NY, USA
3 Northern Territory Department of Primary Industries, Fisheries and Mines, Berrimah Veterinary Laboratories, Berrimah, Northern Territory 0801, Australia

Correspondence
Chris Cowled
chris.cowled{at}csiro.au

Stretch Lagoon orbivirus (SLOV) was isolated in 2002 from pooled Culex annulirostris mosquitoes collected at Stretch Lagoon, near the Wolfe Creek national park in the Kimberley region of Western Australia. Conventional serological tests were unable to identify the isolate, and electron microscopy indicated a virus of the genus Orbivirus, family Reoviridae. Here, a cDNA subtraction method was used to obtain approximately one-third of the viral genome, and further sequencing was performed to complete the sequences of segment 1 (viral polymerase) and segment 2 (conserved inner-core protein). Phylogenetic analysis showed that SLOV should be considered a new species within the genus Orbivirus. A real-time RT-PCR test was designed to study the epidemiology of SLOV in the field. Six additional isolates of SLOV were identified, including isolates from four additional locations and two additional mosquito species. Horses, donkeys and goats were implicated as potential vertebrate hosts in a serological survey.

The GenBank/EMBL/DDBJ accession numbers for the sequences of SLOV segments 1 and 2 are EU718676 and EU718677.






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