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Originally published as JGV in Press, 10.1099/vir.0.009621-0 on March 4, 2009 J Gen Virol 90 (2009), 1491-1498; DOI 10.1099/vir.0.009621-0

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Role of anti-lipopolysaccharide factor from the black tiger shrimp, Penaeus monodon, in protection from white spot syndrome virus infection

Sirinit Tharntada1,2, Sirikwan Ponprateep1, Kunlaya Somboonwiwat1, Haipeng Liu2, Irene Söderhäll2, Kenneth Söderhäll2 and Anchalee Tassanakajon1

1 Shrimp Molecular Biology and Genomics Laboratory, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand
2 Department of Comparative Physiology, Uppsala University, 75236 Uppsala, Sweden

Correspondence
Anchalee Tassanakajon
anchalee.k{at}chula.ac.th

The anti-lipopolysaccharide factor (ALF) from the black tiger shrimp, Penaeus monodon, has been shown previously to exhibit a broad spectrum of activity against various strains of bacteria and fungi. Herein, the recombinant ALFPm3 (rALFPm3) protein was examined for its role in the defence against white spot syndrome virus (WSSV) infection in haematopoietic (Hpt) cell cultures of the freshwater crayfish, Pacifastacus leniusculus, as well as in live P. monodon shrimps. Incubation of Hpt cell cultures with a mixture of WSSV and rALFPm3 resulted in a dose-dependent decrease in VP28 gene expression levels, compared with those incubated with WSSV alone, with an rALFPm3 IC50 value lower than 2.5 µM. However, pre-treatment of Hpt cells with 5 µM rALFPm3 showed no induced protection against subsequent WSSV infection, whereas the synthetic crayfish ALF peptide could protect cells at a higher concentration (10 µM). The in vivo role of ALFPm3 was examined by injection of P. monodon with WSSV pre-treated with rALFPm3 protein. The results clearly showed that rALFPm3 was able to reduce WSSV propagation and prolong the survival of shrimps.

A table showing the primers used in this study is available with the online version of this paper.






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