HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Supplementary Tables All Versions of this Article: vir.0.010694-0v1 90/8/2015    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Google Scholar Articles by Misra, V. Articles by Artsob, H. PubMed PubMed Citation Articles by Misra, V. Articles by Artsob, H. Agricola Articles by Misra, V. Articles by Artsob, H.

Detection of polyoma and corona viruses in bats of Canada

¹ Department of Veterinary Microbiology, Western College of Veterinary Medicine, 52 Campus Road, University of Saskatchewan, Saskatoon, SK S7N 5B4, Canada
² National Microbiology Laboratory, Canadian Science Centre for Human and Animal Disease, 1015 Arlington Street, Winnipeg, MB R3E 3R2, Canada
³ Manitoba Conservation, Wildlife and Ecosystem Protection Branch, Box 24, 200 Saulteaux Crescent, Winnipeg, MB R3J 3W3, Canada
⁴ Department of Biology and Centre for Forest Interdisciplinary Research, University of Winnipeg, 515 Portage Avenue, Winnipeg, MB R3B 2E9, Canada
⁵ Centre of Expertise for Rabies, Ottawa Laboratory-Fallowfield, Canadian Food Inspection Agency, 3851 Fallowfield Road, Ottawa, ON K2H 8P9, Canada

Correspondence
Vikram Misra
vikram.misra{at}usask.ca

Several instances of emerging diseases in humans appear to be caused by the spillover of viruses endemic to bats, either directly or through other animal intermediaries. The objective of this study was to detect, identify and characterize viruses in bats in the province of Manitoba and other regions of Canada. Bats were sampled from three sources: live-trapped Myotis lucifugus from Manitoba, rabies-negative Eptesicus fuscus, M. lucifugus, M. yumanensis, M. septentrionalis, M. californicus, M. evotis, Lasionycteris (L.) noctivagans and Lasiurus (Las.) cinereus, provided by the Centre of Expertise for Rabies of the Canadian Food Inspection Agency (CFIA), and L. noctivagans, Las. cinereus and Las. borealis collected from a wind farm in Manitoba. We attempted to isolate viruses from fresh tissue samples taken from trapped bats in cultured cells of bat, primate, rodent, porcine, ovine and avian origin. We also screened bat tissues by PCR using primers designed to amplify nucleic acids from members of certain families of viruses. We detected RNA of a group 1 coronavirus from M. lucifugus (3 of 31 animals) and DNA from an as-yet undescribed polyomavirus from female M. lucifugus (4 of 31 animals) and M. californicus (pooled tissues from two females).

The GenBank/EMBL/DDBJ accession number of the complete nucleotide sequence of the Myotis polyomavirus and deduced amino acid sequence is FJ188392.

Three supplementary tables showing primer sequences, background information on bat specimens and GenBank accession numbers of the sequences analysed in this study are available with the online version of this paper.