J Gen Virol
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Originally published as JGV in Press, 10.1099/vir.0.012120-0 on June 3, 2009 J Gen Virol 90 (2009), 2097-2106; DOI 10.1099/vir.0.012120-0

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N-Linked glycans on dengue viruses grown in mammalian and insect cells

Kari Hacker1, Laura White1,2 and Aravinda M. de Silva1

1 Department of Microbiology and Immunology, University of North Carolina School of Medicine, Chapel Hill, NC 27599, USA
2 Carolina Vaccine Institute, University of North Carolina School of Medicine, Chapel Hill, NC 27599, USA

Correspondence
Aravinda M. de Silva
desilva{at}med.unc.edu

This study compared the ability of mosquito and mammalian cell-derived dengue virus (DENV) to infect human dendritic cell-specific ICAM3-grabbing non-integrin (DC-SIGN)-expressing cells and characterized the structure of envelope (E) protein N-linked glycans on DENV derived from the two cell types. DENVs derived from both cell types were equally effective at infecting DC-SIGN-expressing human monocytes and dendritic cells. The N-linked glycans on mosquito cell-derived virus were a mix of high-mannose and paucimannose glycans. In virus derived from mammalian cells, the N-linked glycans were a mix of high-mannose and complex glycans. These results indicate that N-linked glycans are incompletely processed during DENV egress from cells, resulting in high-mannose glycans on viruses derived from both cell types. Studies with full-length and truncated E protein demonstrated that incomplete processing was most likely a result of the poor accessibility of glycans on the membrane-anchored protein.






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