J Gen Virol
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Originally published as JGV in Press, 10.1099/vir.0.015198-0 on October 21, 2009 J Gen Virol 91 (2010), 452-462; DOI 10.1099/vir.0.015198-0

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The genome of cyprinid herpesvirus 3 encodes 40 proteins incorporated in mature virions

B. Michel1, B. Leroy2, V. Stalin Raj1, F. Lieffrig3, J. Mast4, R. Wattiez2, A. F. Vanderplasschen1 and B. Costes1

1 Immunology-Vaccinology (B43b), Department of Infectious and Parasitic Diseases, Faculty of Veterinary Medicine, University of Liège, B-4000 Liège, Belgium
2 Proteomic and Protein Biochemistry (Pentagone), University of Mons, B-7000 Mons, Belgium
3 CER Groupe, rue du Carmel 1, B-6900 Marloie, Belgium
4 Department Biocontrole, Research Unit Electron Microscopy, Veterinary and Agrochemical Research Centre, VAR-CODA-CERVA, Groeselenberg 99, B-1180 Ukkel, Belgium

Correspondence
A. F. Vanderplasschen
A.vdplasschen{at}ulg.ac.be

Koi herpesvirus, also known as cyprinid herpesvirus 3 (CyHV-3), is the aetiological agent of an emerging and mortal disease in common and koi carp. CyHV-3 virions present the characteristic morphology of other members of the order Herpesvirales, being composed of an envelope, a capsid containing the genome and a tegument. This study identified CyHV-3 structural proteins and the corresponding encoding genes using liquid chromatography tandem mass spectrometry-based proteomic approaches. In addition, exponentially modified protein abundance index analyses were used to estimate the relative abundance of the identified proteins in CyHV-3 virions. These analyses resulted in the identification of 40 structural proteins, which were classified based on bioinformatic analyses as capsid (three), envelope (13), tegument (two) and unclassified (22) structural proteins. Finally, a search for host proteins in purified CyHV-3 virions indicated the potential incorporation of up to 18 distinct cellular proteins. The identification of the proteins incorporated into CyHV-3 virions and determination of the viral genes encoding these proteins are key milestones for further fundamental and applied research on this virus.






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