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Expression and processing of human immunodeficiency virus type 1 gp160 using the vesicular stomatitis virus New Jersey serotype vector system

¹ University of Western Ontario;
² The University of Western Ontario

³ E-mail: cykang{at}uwo.ca

The Indiana serotype of Vesicular Stomatitis Virus (VSV_IND), but not the New Jersey serotype (VSV_NJ), has been widely used as the gene expression vector. In terms of prime-boost based vaccine strategies, it would be desirable to use two different serotypes of VSV to avoid immunity against the priming viral vector. We report here that we applied VSV_NJ vector system for expression of the env gene of human immunodeficiency virus type 1 (HIV-1). HIV-1 env gene was inserted into VSV_NJ vector system at two different sites: between the P and M genes (NP-gp160-MGL) and between the G and L genes (NPMG-gp160-L). The HIV-1 env gene product, gp160, was efficiently expressed and processed in cells infected with either of these two recombinant VSV-HIV-1_gp160 viruses. In this study, we have discussed the applicability of the VSV_NJ vector system for foreign gene expression.

Received 21 November 2008; accepted 29 January 2009.