J Gen Virol Email Content Delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Published online ahead of print on 4 March 2009 as doi:10.1099/vir.0.009274-0
Journal of General Virology 2009;90:1359.

A more recent version of this article appeared on June 1, 2009 J Gen Virol (2009), DOI 10.1099/vir.0.009274-0
© 2009 Society for General Microbiology
This Article
Right arrow Full Text (Papers in Press[PDF])
Right arrow All Versions of this Article:
vir.0.009274-0v1
90/6/1359    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ratra, R.
Right arrow Articles by Lal, S. K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ratra, R.
Right arrow Articles by Lal, S. K.
Agricola
Right arrow Articles by Ratra, R.
Right arrow Articles by Lal, S. K.

The ORF3 protein of Hepatitis E virus interacts with the b beta chain of fibrinogen resulting in decreased fibrinogen secretion from HuH-7 cells

Ruchi Ratra, Anindita Kar-Roy and Sunil K. Lal1

ICGEB

1 E-mail: sunillal{at}icgeb.res.in

The ORF3 protein of Hepatitis E virus (HEV), the precise cellular functions of which remain obscure, was used in a yeast two-hybrid screen to identify its cellular binding partners. One of the identified interacting partners was fibrinogen B beta protein. The ORF3-fibrinogen B beta interaction was verified by co-immunoprecipitation and fluorescence resonance energy transfer in mammalian cells. Fibrinogen is a hepatic acute phase protein and serves as a central molecule that maintains host homeostasis and hemostasis during an acute phase response. Metabolic labeling of ORF3 transfected HuH-7 cells showed that the secreted as well as intracellular levels of fibrinogen were decreased in these cells compared to vector transfected controls. Northern hybridization and reverse transcription-PCR analyses revealed that the mRNA levels of all three chains of fibrinogen, A alpha, B beta and gamma, were transcriptionally down-regulated in ORF3 transfected cells. The constitutive expression of fibrinogen genes can be significantly up-regulated by IL-6, an important mediator of liver specific gene expression during an acute phase response. Transcription of fibrinogen genes after IL-6 stimulation was lesser in ORF3 expressing cells compared to controls. This report adds one more biological function to and advances our understanding of the cellular role of the ORF3 protein of HEV. The possible implications of these findings in the virus life cycle have been discussed.

Received 1 December 2008; accepted 19 February 2009.




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 2009 by the Society for General Microbiology.