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Published online ahead of print on 4 March 2009 as doi:10.1099/vir.0.009472-0
Journal of General Virology 2009;90:1262.

A more recent version of this article appeared on May 1, 2009 J Gen Virol (2009), DOI 10.1099/vir.0.009472-0
© 2009 Society for General Microbiology
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Analysis of promoter activity among selected Cotesia plutellae bracovirus genes

Jae Young Choi1, Soo-Jin Kwon2, Jong Yul Roh3, Tae-Jin Yang2, Ming Shun Li3, Beom-Seok Park2, Yonggyun Kim4, Soo-Dong Woo5, Byung Rae Jin6 and Yeon Ho Je3,7

1 Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 151-742, Kore;
2 National Institute of Agricultural Biotechnology, Rural Development Administration, Suwon 441-707, K;
3 Department of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National U;
4 Department of Agricultural Biology, Andong National University, Andong 760-749, Korea;
5 Department of Plant Medicine, College of Agriculture, Life and Environment Sciences, Chungbuk Nation;
6 College of Natural Resources and Life Science, Dong-A University, Busan 604-714, Korea

7 E-mail: btrus{at}snu.ac.kr

In a previous study we cloned 27 discrete genome segments of Cotesia plutellae bracovirus (CpBV), and provided the complete nucleotide sequences and annotation. Seven putative coding regions were predicted from one of the largest segments, CpBV-S30. The activity of promoters associated with six predicted ORFs from this segment were investigated using both transient and baculovirus expression assays with EGFP as reporter gene. CpBV promoters showed activity earlier than the polyhedrin promoter, and the activity of some of these promoters was superior to that of the AcMNPV ie-1 promoter in the baculovirus expression assays. The promoter of ORF3004 showed the highest level of activity in insect cells, exhibiting 24% of activity obtained with the AcMNPV polyhedrin promoter in Sf9 cells. In Spodoptera exigua larvae, the ORF3006 promoter showed the highest activity, with about 35% of activity measured with the polyhedrin promoter. In addition, analysis of promoter of ORF3006 revealed that the region between -382 and -422 from the translation start point was critical for activity of this promoter. These results suggest that the CpBV-S30 promoters characterized here could be useful tools in a variety of biotechnological applications such as gene expression analyses and insecticide development.

Received 7 December 2008; accepted 16 January 2009.




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