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Published online ahead of print on 4 March 2009 as doi:10.1099/vir.0.009704-0
Journal of General Virology 2009;90:1724.

A more recent version of this article appeared on July 1, 2009 J Gen Virol (2009), DOI 10.1099/vir.0.009704-0
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Comparison of vesicular stomatitis virus pseudotyped with the S proteins from a porcine and a human coronavirus

Christel Schwegmann-Wessels1,5, Jörg Glende1, Xiaofeng Ren2, Xiuxia Qu3, Hongkui Deng3, Luis Enjuanes4 and Georg Herrler1

1 University of Veterinary Medicine Hannover;
2 Northeast Agricultural University;
3 Peking University;
4 Centro Nacional de Biotecnología, CSIC

5 E-mail: christel.schwegmann{at}tiho-hannover.de

The surface proteins S of SARS-CoV and transmissible gastroenteritis virus (TGEV) were compared for their ability to mediate infection of viral pseudotypes based on vesicular stomatitis virus (VSV). The cell tropism of the respective pseudotypes corresponded to the tropism of the viruses from which the S protein was derived. Higher infectivity values were obtained with SARS-CoV-S than with the TGEV S protein. Differences were observed with respect to the importance of the cytoplasmic tail and the membrane anchor of the S proteins. In the case of the SARS-CoV S protein, truncation of the cytoplasmic tail resulted in an increased infectivity. For the TGEV S protein, the inactivation of an intracellular retention signal in the cytoplasmic tail was required. Exchange of the membrane anchor of the S proteins led to a low infection efficiency. Our results indicate that related glycoproteins may show substantial differences in their ability to mediate pseudotype infection.

Received 16 December 2008; accepted 3 March 2009.




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