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Published online ahead of print on 8 April 2009 as doi:10.1099/vir.0.010330-0
Journal of General Virology 2009;90:1820.

A more recent version of this article appeared on August 1, 2009 J Gen Virol (2009), DOI 10.1099/vir.0.010330-0
© 2009 Society for General Microbiology
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Full-length genomic sequences for HCV subtypes 4b, 4c, 4d, 4g, 4k, 4l, 4m, 4n, 4o, 4p, 4q, 4r, and 4t

Li Chunhua1, Ling Lu1,7, Xianghong Wu2, Chuanxi Wang3, Phil Bennett4, Teng Lu5 and Donald Murphy6

1 University of Utah;
2 Kunming Teacher's college;
3 Guangzhou Blood Center, Guangzhou, Guangdong, China;
4 Micropathology Ltd, University of Warwick Science Park, Coventry, United Kingdom;
5 University of Georgia, GA;
6 Institut national de sante publique du Quebec, Laboratoire de sante publique du Quebec

7 E-mail: ling.lu{at}hsc.utah.edu

In this study, we characterized the full-length genomic sequences of 13 distinct HCV genotype 4 isolates/subtypes: QC264/4b, QC381/4c, QC382/4d, QC193/4g, QC383/4k, QC274/4l, QC249/4m, QC97/4n, QC93/4o, QC139/4p, QC262/4q, QC384/4r, and QC155/4t. These were amplified, using RT-PCR, from the sera of patients now residing in Canada, 11 of which were African immigrants. The resulting genomes varied between 9421-9475 nucleotides in length and each contains a single ORF of 9018-9069 nucleotides. The sequences showed nucleotide similarities between 77.3-84.3% in comparison to subtypes 4a (Y11604) and 4f (EF589160 [GenBank] ) and between 70.6-72.8% in comparison to genotype 1 (M62321/1a, M58335/1b, D14853/1c, and 1?/AJ851228) reference sequences. These similarities were often higher than those currently defined by HCV classification criteria for subtype (75.0-80.0%) and genotype (67.0-70.0%) division, respectively. Further analyses of the complete and partial E1 and partial NS5B sequences confirmed these 13 'provisionally-assigned-subtypes'.

Received 13 January 2009; accepted 7 April 2009.




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