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Published online ahead of print on 4 March 2009 as doi:10.1099/vir.0.010397-0
Journal of General Virology 2009;90:1641.

A more recent version of this article appeared on July 1, 2009 J Gen Virol (2009), DOI 10.1099/vir.0.010397-0
© 2009 Society for General Microbiology
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Characterization of a virion occlusion defective Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV) mutant lacking the p26, p10 and p74 genes

Lihua Wang, Tamer Z. Salem, Dean J. Campbell, Colin M. Turney, C. M. Senthil Kumar and Xiao-Wen Cheng1

Miami University

1 E-mail: chengx{at}muohio.edu

The nucleopolyhedroviruses (NPVs) of the viral family Baculoviridae are insect specific viruses that have the potential to control insect pests in agriculture and forestry. NPVs are occluded in polyhedral shaped occlusion bodies (OBs). Polyhedra protect virions from inactivation in the environment as well as assist virions in horizontal transmission in the insect population. The process of virion occlusion in the polyhedra is unknown, and the genes that regulate the virion occlusion process have not well been investigated yet. An Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV) mutant that has a 2,136 bp DNA sequence deletion including p26, p10 and p74 genes (AcDef) has been isolated. No virions have been detected in the polyhedra of AcDef. Restoration of all the missing sequences into AcDef led to proper virion occlusion. Individual gene deletion of either p10 or p26 could not abolish virion occlusion in the polyhedra of AcMNPV, but p10 gene deletion reduced virion occlusion efficiency more than 3-fold compared to the wild type AcMNPV. Previous studies by other research groups on gene deletion of AcMNPV p74 suggested p74 is a per os infectivity factor, and deletion of the p74 gene did not eliminate virion occlusion. Collectively, the three genes (p26, p10 and p74) may be concerted to regulate the virion occlusion process. Therefore, all three genes of p26, p10 and p74 are required for proper virion occlusion in the polyhedra of AcMNPV.

Received 17 January 2009; accepted 26 February 2009.




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