Data Collection for:
Law et al. (2002). Journal of General Virology, 83,
part 1, pp.
209222.
Supplementary Data
The following material is available only in JGV Online:
Immunoblot of purified IMV and EEV using Rb-WR2 Ab. Purified IMV and EEV
(10 µg each) were resolved by SDSPAGE (12 % gel) and transferred to a
nitrocellulose membrane. Virus-specific proteins were detected with Rb-WR2
IgG (0.25 µg/ml) and horseradish peroxidase-conjugated goat anti-rabbit IgG
(diluted 1/2000, Sigma) using an ECL Western Blotting detection system
(Amersham Pharmacia Biotech). Common bands represent the immunodominant IMV
and core proteins shared by IMV and EEV. Potential EEV-specific proteins
are indicated by arrows.