Data Collection for:
Rodger & Smith (2002). Journal of General Virology, 83,
part 2, pp. 323–332.
Supplementary data
The following material is available only in JGV Online:
PCR analysis of the vB5R-EGFP genome. BS-C-1 cells were infected with WR
WT, vB5R-EGFP or vDB5R at 1 p.f.u. per cell and harvested at 24 h p.i. Virus
DNA was isolated by phenol–chloroform extraction followed by ethanol
precipitation
and used as template for PCR using oligonucleotide primers that (a) flanked
the complete B5R ORF (5´ TCATTTAAGCTTCCTTCTTTCGTGAAATGC 3´ and 5´
GTACTCAAGCTTGCTTACAGAAACATCGCGTT 3´), (b) amplified the EGFP ORF (5´
GTGAGCAAGGGCGAGGAG 3´ and 5´ CTTGTACAGCTCGTCCAT 3´) or (c) amplified
only SCR domains 2 to 4 of B5R (5´ TGTGCACAGTTTCTGATTACAT 3´ and 5´
TCGTACACATATTGGGAGTAC 3´). PCR products were resolved by electrophoresis
on a 0.8 % agarose gel, stained with ethidium bromide and visualized under
UV illumination. The positions of dsDNA markers are shown in kb.
