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Data Collection for:

Mason et al. (2003). Journal of General Virology, 84, part 6, pp. 1583–1593.


Supplementary Data


The following material is available only in JGV Online:



Table 1. Oligonucleotide primers used for RT-PCR and sequencing

Number Purpose*/sense† Sequence‡ Position§
P614 PCR/sense tatcccgggttcTTGAAAGGGGGCGTTAGGG S (1–19)
P599 SEQ/sense CACYYTCCGCCTACTTGGTCGT S (208–229)
P55 SEQ/antisense GCCCAAGACAGCGCTAACG S (227–245)
P606 PCR/antisense cgtgcctaGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGAAAGGTGG S (359–378)
P609 PCR/antisense tatcctaGGGGGGGGGGGGGGGGT S (368–378)
P600 PCR/sense CCAAGTTTTTACCGCCTTTCCCGG PK (377–400)
P166 SEQ/sense GGAGGCCTTGTACAAACACGATC cre (606–628)
P4 SEQ/antisense CCTCTAGACCAGGAAAGACC IRES (679–698)
P603 PCR/sense TGGCCGCGGGAACTCCTCCTTG IRES (785–806)
P548 SEQ/antisense CCTGAAGGGCATCCTTAGCC IRES (937–956)
P607¦¦ PCR/sense ACCTCCRACGGGTGGTACGC L (1554–1573)
P536 SEQ/sense YRACGGGTGGTACGCGAT L (1559–1576)
P538 SEQ/sense GGRGTSACKTACGGGTAC VP2 (2040–2057)
P539 SEQ/sense CTMCCRACTGACCACAAAGG VP2 (2196–2215)
P540 SEQ/sense YAAGGTSTATGCCAACAT VP2 (2540–2257)
P541 SEQ/sense ATYAAYCTSCACTTCATGTT VP3 (2916–2935)
P498 PCR/sense AATTACACATGGCAAGGCCGACGG VP3 (3167–3190)
P617 PCR/sense CCATCAAAGCCACTCGGG VP1 (3763–3780)
P514 SEQ/sense CCAACCCCGGGCCTTTCTTCTTCTCTGACG 2B (3940–3969)
P500# PCR/antisense GACATGTCCTCCTGCATCTG 2B (4008–4027)
P618 SEQ/sense TGATTCTTGCCATCCGCG 2C (4477–4494)
P534 SEQ/sense CAAGCGTGTTTGAAGAGCGG 2C (4635–4654)
P502 SEQ/sense CAGATATCAATTCCTTCCCAAAAGGCT 3A (5364–5390)
P503 SEQ/antisense cccggatccaTATGGTCCTTCAGCTTGTGG 3A (5814–5833)
P512 SEQ/sense gagagatctggacatatgCCGGTCCTCTAGAGCGTCAAA 3B (5836–5856)
P515 SEQ/sense ACCCCCGTCGTCGGCGTGATCAA 3C (6375–6397)
P367 SEQ/sense TGCTGCTGACTACGCGTC 3D (6908–6925)
P517 SEQ/sense GTGATGGCCTCGAAGACCCTCG 3D (7881–7902)
P530 SEQ/antisense GCAGGTAAAGTGATCTGTAGCTTGG 3D (8034-8058)
P15 PCR/antisense ggcggccgcTTTTTTTTTTTTTTT poly(A)

* PCR indicates primers used for PCR (also RT, in the case of antisense primers). SEQ indicates primers used for sequencing only. Not all primers were utilized for all genomes.

† Primer's sense on the genome.

‡ sequence of the oligonucleotide, 5´ to 3´, standard abbreviations, lowercase bases do not match genomes but were needed for cDNA manipulations for other purposes.

§ Functional element followed by nucleotide numbers, in parentheses for the PanAsia genome numbered as shown in Fig.1 of the printed manuscript.

¦¦ Also known as L-463F [#260, Samuel (2001)].

Also known as O-1C564 [#260, Samuel (2001)].

# Also known as NK61 [#260, Samuel (2001)].




Supplementary Fig. 1

Fig. 1. Genome scanning comparisons of genomic sequence data of O/UKG/35/2001 versus FMDV A, C, Asia1 and SAT2 isolates. The origins of the sequence data are in the text and figure legends of the printed manuscript.




Supplementary Fig. 2

Fig. 2. Neighbour-joining tree (outgroup-rooted on SAT2/KEN/3/57) showing relationships among the PanAsia viruses and other FMDV strains created based on VP1 sequence data. The origins of the sequence data are in the text and figure legends of the printed manuscript. Confidence limits shown at each branch-point indicate the number of times (out of 1000) that bootstrap replicates produced this branch point.






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