| Amino acid sequence alignment of baculovirus LEF-8 sequences. LEF-8 sequences were aligned using DS Gene 1.0 from Accelrys Inc. Consensus sequences are shown on the bottom lines. Predicted polypeptide sequences were obtained from GenBank from the following baculoviruses and accession numbers: Autographa californica MNPV (AcMNPV, L22858); Bombyx mori NPV (BmNPV, L33180); Orgyia pseudotsugata MNPV (OpMNPV, U75930); Spodoptera exigua MNPV (SeMNPV, AF169823); Lymantria dispar MNPV (LdMNPV, AF081810); Culex nigripalpus NPV (CuniNPV, AF403738); Helicoverpa armigera SNPV (HaSNPV, AF271059); Helicoverpa zea SNPV (HzSNPV, AF334030); Epiphyas postvittana MNPV (EppoMNPV, AY043265); Spodoptera litura (SpltMNPV, AF325155); Mamestra configurata NPV isolate 90/2 [MacoNPV (90/2), AF467808]; Mamestra configurata NPV isolate 96B [MacoNPV (96B), AY126275]; Cydia pomonella granulovirus (CpGV, U53466); Plutella xylostella GV (PxGV, AF270937); Xestia c-nigrum GV (XcGV, AF162221); Phthorimaea operculetta GV (PhopGV, AF499596). ts, Position of the temperature-sensitive mutation in BmNPV lef-8, tsS1. Asterisks below the consensus sequence in Fig. 7 indicate where point mutations were introduced in the conserved RNA polymerase motif. |
